Compatible Cohesive Ends

   

Enzyme

Ligated To

Recleaved By

Acc65 I
(G/GTACC)

Ban I (G/GTACC)

Acc65 I, Ban I, Kpn I, Nla IV, Rsa I 

BsiW I, BsrG I

Rsa I

Acc I

(GT/CGAC)

Aci I, Acl I, BsaH I (GR/CGYC), HinP1 I, Hpa II, Nar I

--

(GT/CGAC)

Cla I, BstB I, Taq I

Taq I

Aci I
(C/CGC)

Acc I (GT/CGAC), Acl I, Cla I, BstB I, Taq I

--

BsaH I (GR/CGCC), HinP1 I, Nar I

Aci I

Hpa II

Hpa II

Acl I
(AA/CGTT)

Acc I (GT/CGAC), Aci I, Cla I, BstB I, HinP1 I, Hpa II, Nar I, Taq I

--

Afl III

(A/CGCGT)

Asc I, BssH II

BstU I, Hha I

(A/CATGT)

BspH I, Nco I, Pci I

Nla III

(A/CGCGT)

Mlu I

Afl III, BstU I, Mlu I

Age I
(A/CCGGT)

Ava I (C/CCGGG), Xma I

Hpa II, Nci I, ScrF I

BsaW I, BspE I

BsaW I, Hpa II

BsrF I (A/CCGGT), SgrA I (CA/CCGGTG)

Age I, BsaW I, BsrF I, Hpa II

NgoM IV

BsrF I, Hpa II

Apa I
(GGGCC/C)

Ban II (GGGCC/C), Bsp1286 I (GGGCC/C)

Apa I, Ban II, Bsp120 I, Bsp1286 I, Hae III, Nla IV, Sau96 I

ApaL I
(G/TGCAC)

Sfc I (C/TGCAG)

Bsg I

Apo I

(A/AATTY)

EcoR I

Apo I, Tsp509 I

(G/AATTY)

EcoR I

Apo I, EcoR I, Tsp509 I

(R/AATTY)

Mfe I, Tsp509 I

Tsp509 I

Asc I
(GG/CGCGCC)

Afl III (A/CGCGT), Mlu I

BstU I, Hha I

BssH II

BssH II, BstU I, Cac8 I, Hha I

Ase I
(AT/TAAT)

Bfa I, Csp6 I, Nde I

--

Mse I

Mse I

AsiS I
(GCGAT/CGC)

BsiE I (CGAT/CG)

Dpn II, Pvu I

Pac I

Mse I

Pvu I

Dpn II, Pvu I

Ava I

(C/CCGGG)

Age I, BsaW I, BspE I, BsrF I (R/CCGGY), NgoM IV, SgrA I (CR/CCGGYG)

Hpa II, Nci I, ScrF I

(C/TCGAG)

Xho I

Ava I, Taq I, Xho I

(C/TCGAG)

Sal I

Taq I

(C/CCGGG)

Xma I

Ava I, BsaJ I, Hpa II, Nci I, ScrF I, Sma I

Ava II
(G/GWCC)

PpuM I (RG/GACCY)

Ava II, Nla IV, Sau96 I

Rsr II

Ava II, Sau96 I

PpuM I (RG/GTCCY)

Ava II, BsmF I, Nla IV, Sau96 I

Avr II
(C/CTAGG)

Nhe I, Spe I, Xba I

Bfa I

Sty I (C/CTAGG)

Avr II, Bfa I, BsaJ I, Sty I

BamH I
(G/GATCC)

Bcl I, Dpn II

Alw I, Dpn II

Bgl II, BstY I (R/GATCY)

Alw I, BstY I, Dpn II

BstY I (G/GATCC)

Alw I, BamH I, BstY I, Dpn II, Nla IV

Ban I

(G/GTACC)

Acc65 I

Acc65 I, Ban I, Kpn I, Nla IV, Rsa I

(G/GCGCC)

Kas I

Ban I, BsaH I, Hae II, Hha I, Kas I, Nar I, Nla IV

(G/GTACC)

BsiW I, BsrG I

Rsa I

Ban II

(GGGCC/C)

Apa I, Bsp1286 I (GGGCC/C)

Apa I, Ban II, Bsp1286 I, Hae III, Nla IV, Sau96 I

(GAGCT/C)

Bsp1286 I (GAGCT/C), Sac I

Alu I, Ban II, BsiHKA I, Bsp1286 I, Sac I

Bcl I
(T/GATCA)

BamH I, BstY I (R/GATCY)

Alw I, Dpn II

Bgl II, Mbo II

Dpn II

Bfa I
(C/TAG)

Ase I, Csp6 I, Mse I, Nde I

--

Bgl II
(A/GATCT)

BamH I, BstY I (R/GATCY)

Alw I, BstY I, Dpn II

Bcl I, Dpn II

Dpn II

BsaH I

(GR/CGYC)

Acc I (GT/CGAC), Cla I, BstB I, Taq I

--

(GA/CGYC)

Aci I, HinP1 I

Hga I

(GG/CGYC)

Aci I, HinP1 I

Hha I

(GG/CGYC)

Hpa II

Aci I

(GA/CGYC)

Nar I

BsaH I, Hga I

(GG/CGYC)

Nar I

Ban I, BsaH I, Hae II, Hha I, Nar I, Nla IV

BsaW I
(W/CCGGW)

Age I, BsrF I (R/CCGGY), SgrA I (CR/CCGGYG)

Age I, BsaW I, BsrF I, Hpa II

Ava I (C/CCGGG), Xma I

Hpa II, Nci I, ScrF I

BspE I

BsaW I, BspE I, Hpa II

BsrF I (R/CCGGY), NgoM IV

BsrF I, Hpa II

NgoM IV

Hpa II

BsiE I

(CGAT/CG)

Pac I

Mse I

(CGAT/CG)

Pvu I

BsiE I, Dpn II, Pvu I

(CGGC/CG)

Sac II

Aci I

BsiHKA I
(GTGCA/C)

Bsp1286 I (GTGCA/C)

BsiHKA I, Bsp1286 I

Bsp1286 I (GAGCA/C)

BsiHKA I, Bsp1286 I

Bsp1286 I (GAGCT/C), Sac I

Alu I, Ban II, BsiHKA I, Bsp1286 I, Sac I

Nsi I

--

Pst I, Sbf I

Bsg I

BsiW I
(C/GTACG)

Acc65 I, Ban I (G/GTACC), BsrG I

Rsa I

Bsp1286 I

(GGGCC/C)

Apa I, Ban II (GGGCC/C)

Apa I, Ban II, Bsp1286 I, Hae III, Nla IV, Sau96 I

(GTGCA/C)

BsiHKA I

ApaL I, BsiHKA I, Bsp1286 I

(GGGCC/C)

Ban II (GGGCC/C)

Ban II, Bsp1286 I

(GAGCT/C)

Ban II (GAGCT/C), BsiHKA I, Sac I

Alu I, Ban II, BsiHKA I, Bsp1286 I, Sac I

(GWGCW/C)

BsiHKA I

BsiHKA I, Bsp1286 I

(GTGCA/C)

Nsi I

--

(GTGCA/C)

Pst I, Sbf I

Bsg I

BspE I
(T/CCGGA)

Age I, BsaW I, BsrF I (R/CCGGY), SgrA I (CR/CCGGYG)

BsaW I, Hpa II

Ava I (C/CCGGG), Xma I

Hpa II, Nci I, ScrF I

BsaW I

BsaW I, BspE I, Hpa II

BsrF I (R/CCGGY), NgoM IV

Hpa II

BspH I
(T/CATGA)

Afl III (A/CATGT), Nco I, Sty I (C/CATGG)

Nla III

BsrF I

(A/CCGGY)

Age I, BsaW I

Age I, BsaW I, BsrF I, Hpa II

(G/CCGGY)

Age I, BsaW I, NgoM IV

BsrF I, Hpa II

(R/CCGGY)

Ava I (C/CCGGG), Xma I

Hpa II, Nci I, ScrF I

(A/CCGGY)

BsaW I, BspE I

BsaW I, Hpa II

(R/CCGGY)

BsaW I, BspE I

Hpa II

(G/CCGGY)

NgoM IV

BsrF I, Cac8 I, Hpa II, Nae I

(CR/CCGGYG)

SgrA I

BsrF I, Hpa II

BsrG I
(T/GTACA)

Acc65 I, Ban I (G/GTACC), BsiW I

Rsa I

BssH II
(G/CGCGC)

Afl III (A/CGCGT), Mlu I

BstU I, Hha I

Asc I

BssH II, BstU I, Cac8 I, Hha I

BstB I
(TT/CGAA)

Acc I (GT/CGAC), Cla I, Taq I

Taq I

Aci I, Acl I, BsaH I (GR/CGYC), HinP1 I, Hpa II, Nar I

--

BstY I

(A/GATCY)

BamH I, Bgl II

Alw I, BstY I, Dpn II

(G/GATCY)

BamH I

Alw I, BamH I, BstY I, Dpn II, Nla IV

(R/GATCY)

Bcl I, Dpn II

Dpn II

(G/GATCY)

Bcl I, Dpn II

Alw I, Dpn II

(A/GATCY)

Bgl II

Bgl II, BstY I, Dpn II

Cla I
(AT/CGAT)

Acc I (GT/CGAC), BstB I, Taq I

Taq I

Aci I, Acl I, BsaH I (GR/CGYC), HinP1 I, Hpa II, Nar I

--

Dpn II/Mbo I/
Sau3A I (/GATC)

BamH I, BstY I (R/GATCC)

Alw I, Dpn II

Bcl I, Bgl II, BstY I (R/GATCY)

Dpn II

Eae I

(Y/GGCCR)

PspOM I

Hae III, Sau96 I

(C/GGCCR)

Eag I

BsiE I, Eae I, Eag I, Hae III

(T/GGCCR)

Eag I

Eae I, Hae III

(C/GGCCR)

Not I

Aci I, BsiE I, Eae I, Eag I, Fnu4H I, Hae III

(T/GGCCR)

Not I

Aci I, Eae I, Fnu4H I, Hae III

Eag I
(C/GGCCG)

PspOM I

Hae III, Sau96 I

Eae I (Y/GGCCR)

Eae I, Hae III

Eae I (C/GGCCG)

BsiE I, Eae I, Eag I, Hae III

Not I

Aci I, BsiE I, Eae I, Eag I, Fnu4H I, Hae III

EcoR I
(G/AATTC)

Apo I (G/AATTC)

Apo I, EcoR I, Tsp509 I

Apo I (R/AATTY)

Apo I, Tsp509 I

Mfe I, Tsp509 I

Tsp509 I

HinP1 I
(G/CGC)

Acc I (GT/CGAC), Acl I, Cla I, BstB I, Taq I

--

Aci I, BsaH I (GR/CGCC), Nar I

Hha I

BsaH I (GR/CGTC)

Hga I

Hpa II

Aci I

Hpa II/Msp I
(C/CGG)

Acc I (GT/CGAC), Acl I, Cla I, BstB I, Taq I

--

Aci I, BsaH I (GR/CGCC), HinP1 I, Nar I

Aci I

Kas I
(G/GCGCC)

Ban I (G/GCGCC)

Ban I, BsaH I, Hae II, Hha I, Kas I, Nar I, Nla IV

Mfe I
(C/AATTG)

Apo I (R/ATTTY), EcoR I, Tsp509 I

Tsp509 I

Mlu I
(A/CGCGT)

Afl III (A/CGCGT)

Afl III, BstU I, Mlu I

Asc I, BssH II

BstU I, Hha I

Mse I
(T/TAA)

Ase I

Mse I

Bfa I, Csp6 I, Nde I

--

Nar I
(GG/CGCC)

Acc I (GT/CGAC), Acl I, Cla I, BstB I, Taq I

--

Aci I, HinP1 I

Hha I

BsaH I (GR/CGCC)

Ban I, BsaH I, Hae II, Hha I, Nar I, Nla IV

BsaH I (GR/CGTC)

BsaH I, Hga I

Hpa II

Aci I

Nco I
(C/CATGG)

Afl III (A/CATGT), BspH I

Nla III

Sty I (C/CATGG)

BsaJ I, Nco I, Nla III, Sty I

Pci I

Nla III

Nde I
(CA/TATG)

Ase I, Bfa I, Csp6 I, Mse I

--

NgoM IV
(G/CCGGC)

Age I, BsaW I, BsrF I (R/CCGGY), SgrA I

BsrF I, Hpa II

Ava I (C/CCGGG), Xma I

Hpa II, Nci I, ScrF I

BsaW I, BspE I

Hpa II

BsrF I (R/CCGGC), SgrA I

BsrF I, Cac8 I, Hpa II, Nae I

Nhe I
(G/CTAGC)

Avr II, Spe I, Sty I (C/CTAGG), Xba I

Bfa I

Nla III
(CATG/)

Sph I, Nsp I

Nla III

Not I
(GC/GGCCGC)

PspOM I

Aci I, Eae I, Fnu4H I, Hae III

Eag I

Aci I, BsiE I, Eae I, Eag I, Fnu4H I, Hae III

Eae I (Y/GGCCR)

Aci I, BsiE I, Eae I, Fnu4H I, Hae III

Nsi I
(ATGCA/T)

BsiHKA I (GTGCA/C), Bsp1286 I (GTGCA/C), Pst I, Sbf I

--

Nsp I
(RCATG/Y)

Nla III, Sph I

Nla III

Pac I
(TTAAT/TAA)

BsiE I (CGAT/CG), Pvu I

Mse I

Pci I
(A/CATGT)

Afl II (A/CATGT), BspH I, Nco I, Sty I (C/CTAGG)

Nla III

PpuM I

(RG/GWCCY)

Ava II, Rsr II

Ava II, Sau96 I

(GG/GTCCY)

Ava II, Rsr II

Ava II, BsmF I, Nla IV, Sau96 I

(GG/GACCY)

Ava II, Rsr II

Ava II, Nla IV, Sau96 I

PspOM I
(G/GGCCC)

Eae I (Y/GGCCR), Eag I

Hae III, Sau96 I

Not I

Aci I, Fnu4H I, Hae III, Sau96 I

Pst I
(CTGCA/G)

BsiHKA I, Bsp1286 I (GTGCA/C)

Bsg I

Nsi I

--

Sbf I

Pst I

Pvu I
(CGAT/CG)

AsiS I

Dpn I, Pvu I

Pac I

Mse I

BsiE I (CGAT/CG)

BsiE I, Dpn II, Pvu I

Rsr II
(CG/GWCCG)

Ava II, PpuM I (RG/GACCY)

Ava II, Sau96 I

PpuM I (RG/GACCY)

Ava II, Nla IV, Sau96 I

PpuM I (RG/GTCCY)

Ava II, BsmF I, Nla IV, Sau96 I

Sac I
(GAGCT/C)

Ban II (GAGCT/C), BsiHKA I, Bsp1286 I (GAGCT/C)

Alu I, Ban II, BsiHKA I, Bsp1286 I, Sac I

Sac II
(CCGC/GG)

BsiE I (CGGC/CG)

Aci I

Sal I
(G/TCGAC)

Ava I (C/TCGAG), Xho I

Taq I

Sbf I
(CCTGCA/GG)

BsiHKA I, Bsp1286 I (GTGCA/C)

Bsg I

Nsi I

--

Pst I

Pst I

Sfc I
(C/TGCAG)

ApaL I

Bsg I

SgrA I
(CR/CCGGYG)

See BsrF I

 

Spe I
(A/CTAGT)

Avr II, Nhe I, Sty I (C/CTAGG), Xba I

Bfa I

Sph I
(GCATG/C)

Nla III, Nsp I

Nla III

Sty I

Avr II

Avr II, Bfa I, BsaJ I, Sty I

(C/CTAGG)

Nhe I, Spe I, Xba I

Bfa I

(C/CATGG)

BspH I

Nla III

Nco I

BsaJ I, Nco I, Nla III, Sty I

Taq I
(T/CGA)

Acc I (GT/CGAC), Cla I, BstB I

Taq I

Aci I, Acl I, BsaH I (GR/CGYC), HinP1 I,Hpa II, Nar I

--

Tsp509 I
(/AATT)

Apo I (R/AATTY), EcoR I, Mfe I

Tsp509 I

Xba I
(T/CTAGA)

Avr II, Nhe I, Spe I, Sty I (C/CTAGG)

Bfa I

Xho I/Tli I
(C/TCGAG

Ava I (C/TCGAG)

Ava I, Taq I, Xho I

Sal I

Taq I

Xma I
(C/CCGGG

Age I, BsaW I, BspE I, BsrF I, NgoM IV, SgrA I

Hpa II, Nci I, ScrF I

Ava I (C/CCGGG)

Ava I, BsaJ I, Hpa II, Nci I, ScrF I, Sma I, Xma I